Effectiveness of Dose Concentration of Ethanol Extract of Iles-iles Tubers on Increasing Number of Macrophage Cells and Weight of Immune Organ Weight in White Rats Wistar Strain

Arfatul Makiyah

Abstract

This study was conducted to test the activity of macrophages, since macrophages play an important role in the immune response and to analyze the phagocytic activity due to the effects of flavonoids and polysaccharides iles iles on the immune response. The purpose of this study was to analyze the effectiveness of dose concentration of ethanol extract iles-iles to the increased number of macrophages and the immune organ weights heavy on white rats Wistar strain. This study is a laboratory experimental study of white wistar strains. This research was conducted at Pharmacology Laboratory of Padjadjaran University of Bandung and conducted in May-June 2017. Wistar strain of white rat of 30 tails is divided into 5 groups. Wistar strain white mice in each treatment were administered orally for 7 days on June 1 to 7, 2017. The effectiveness of the effect parameter dose concentration of ethanol extract iles-iles to the increased number of macrophages and the immune organ weights heavy on white rats Wistar strain microscopically are an increase in the number of macrophages and macroscopically is an increase in the weight of the immune organ, namely the liver, spleen and thymus. Microscopically through macrophage images assessed after 1, 3 and 6 hour inoculation of S. aureus. The results showed that the number of macrophages in the group of ethanol extract iles-iles dose of 220 mg /kg on treatment after 1, 3 and 6 h higher than the group of ethanol extract iles-iles dose of 110 mg / kg and 55 mg/kg ( P <0.05), as well as comparison with the Tragakan and aquadest negative control group (p <0.05). Thus, the ethanol extract of iles-iles (Amorphophallus variabilis Bl.) Effective for concentration dose of ethanol extract of iles-iles 220 mg/kg as an immunostimulant and as effective enhancement of immune response is better compared to the control comparator tragacanth 0,3%.

Key words: Ethanol Extract, Dosage, Number of macrophages, Weight of Immune Organ, Iles-iles Tubers

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